Effect of mechanical stimulation on bone marrow stromal cell–seeded tendon slice constructs: A potential engineered tendon patch for rotator cuff repair. (May 2015)

• Record Type: Journal Article
• Authors: Qin, Ting-Wu; Sun, Yu-Long; Thoreson, Andrew R.; Steinmann, Scott P.; Amadio, Peter C.; An, Kai-Nan; Zhao, Chunfeng
• Is Part Of: Biomaterials. Volume 51(2015)
• Page Start: 43
• Page End: 50
• Publication Date: 2015-05
• Subjects: Bone marrow stromal cells -- Stretch -- Tendon slice -- Tissue engineering; BMSC bone marrow stromal cell -- DTS decellularized tendon slices -- ECM extracellular matrix -- GAPDH glyceraldehyde 3-phosphate dehydrogenase -- IACUC Institutional Animal Care and Use Committee -- PBS phosphate-buffered saline -- RT-PCR reverse-transcription polymerase chain reaction -- UTS ultimate tensile strength; Biomedical materials -- Periodicals; Biocompatible Materials -- Periodicals; Biomatériaux -- Périodiques; 610.28
• DOI: 10.1016/j.biomaterials.2015.01.070
• Languages: English
• ISSNs: 0142-9612

Abstract

Abstract: Cell-based tissue engineered tendons have potential to improve clinical outcomes following rotator cuff repair, especially in large or massive rotator cuff tears, which pose a great clinical challenge. The aim of this study was to develop a method of constructing a functional engineered tendon patch for rotator cuff repair with cyclic mechanical stimulation. Decellularized tendon slices (DTSs) were seeded with BMSCs and subjected to cyclic stretching for 1, 3, or 7 days. The mechanical properties, morphologic characteristics and tendon-related gene expression of the constructs were investigated. Viable BMSCs were observed on the DTS after 7 days. BMSCs penetrated into the DTSs and formed dense cell sheets after 7 days of mechanical stretching. Gene expression of type I collagen, decorin, and tenomodulin significantly increased in cyclically stretched BMSC-DTS constructs compared with the unstrained control group ( P < 0.05). The ultimate tensile strength and stiffness of the cyclically stretched tendon constructs were similar to the unstrained control group ( P > 0.05). In conclusion, mechanical stimulation of BMSC-DTS constructs upregulated expression of tendon-related proteins, promoted cell tenogenic differentiation, facilitated cell infiltration and formation of cell sheets, and retained mechanical properties. The patch could be used as a graft to enhance the surgical repair of rotator cuff tears.