Efficient preparative separation of β‐cypermethrin stereoisomers by supercritical fluid chromatography with a two‐step combined strategy. Issue 6 (25th January 2018)

• Record Type: Journal Article
• Authors: Yan, Yilun; Fan, Jun; Lai, Yecai; He, Jianfeng; Guo, Dong; Zhang, Hui; Zhang, Weiguang
• Is Part Of: Journal of separation science. Volume 41:Issue 6(2018)
• Page Start: 1442
• Page End: 1449
• Publication Date: 2018-01-25
• Subjects: β‐cypermethrin -- chiral separation -- chromatographic conditions -- supercritical fluid chromatography -- two‐step strategy; Separation (Technology) -- Periodicals; Chromatographic analysis -- Periodicals; 543.089
• DOI: 10.1002/jssc.201701138
• Languages: English
• ISSNs: 1615-9306

Abstract

Abstract: An efficient two‐step method has been developed for the separation of β‐cypermethrin stereoisomers by supercritical fluid chromatography with polysaccharide chiral stationary phases. With respect to retention, selectivity, and resolution of β‐cypermethrin, the effects of chiral stationary phases, cosolvents, mobile phases, and column temperature have been studied in detail. Through a two‐step separation, β‐cypermethrin was firstly separated by using a cellulose‐derived chiral stationary phase to obtain two stereoisomeric pairs, and further resolved on an amylose‐based chiral stationary phase to produce four enantiopure stereoisomers. The electronic circular dichroism patterns of the first‐ and the third‐eluted isomers in methanol solution showed the mirror image of each other in the wavelength range 200∼300 nm, indicating that they were a pair of enantiomers. Moreover, the second‐ and the fourth‐eluted isomers were also enantiomers. This proposed two‐step strategy showed low solvent consumption, fast separation speed, and high‐purity, which may provide an effective approach for preparative separation of compounds with multiple chiral centers and difficult‐to‐separate multicomponent samples.