A highly sensitive SERS probe for bisphenol A detection based on functionalized Au@Ag nanoparticles. Issue 47 (15th November 2018)

• Record Type: Journal Article
• Authors: Wang, Chun-Yang; Zeng, Yi; Shen, Ai-Guo; Hu, Ji-Ming
• Is Part Of: Analytical methods. Volume 10:Issue 47(2018)
• Page Start: 5622
• Page End: 5628
• Publication Date: 2018-11-15
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c8ay01966e
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : The presence of BPA reduced the orange-to-grey color change (aggregation degree) of the SERS probe and weakened the enhancement of the Raman signal. Abstract : Bisphenol A (BPA) usually exists in daily plastic products and its harmful effect on human health has been a popular topic. However, many conventional analytical methods need long sample preparation and measurement time, and some BPA biosensors suffer from the limitation of complex probe modification. Herein, we designed a simple SERS-coded aptamer sensor for BPA detection based on the electrostatic interaction between chitosan encapsulated 4-MBA coated Au@Ag NPs and anti-BPA aptamers. The negatively charged nucleic acid anti-BPA aptamer will cause the aggregation of the positively charged noble metal nano-reinforced substrate in acid buffer systems, resulting in a significant enhancement of the Raman signal. The specific binding of BPA with nucleic acid anti-BPA aptamers reduced the degree of aggregation of nanoparticles and weakened the SERS signal. This SERS-coded aptamer sensor had a wide linear range of 0.01–1 ng mL −1 and the limit of detection (LOD) was as low as 2.8 pg mL −1, and the method was successfully applied to detect BPA in bottled water within 15 min. As a rapid, simple, ultrasensitive and highly selective analytical technique for the detection of BPA, this SERS-based method would be important in biomedical diagnostics, environmental monitoring and food safety.