An AceDAN–porphyrin(Zn) dyad for fluorescence imaging and photodynamic therapy via two-photon excited FRET. Issue 12 (23rd October 2018)

• Record Type: Journal Article
• Authors: Zhu, Mengliang; Su, Chaorui; Xing, Peipei; Zhou, Yabin; Gong, Lei; Zhang, Jinghui; Du, Hongwu; Bian, Yongzhong; Jiang, Jianzhuang
• Is Part Of: Inorganic chemistry frontiers. Volume 5:Issue 12(2018)
• Page Start: 3061
• Page End: 3066
• Publication Date: 2018-10-23
• Subjects: Chemistry, Inorganic -- Periodicals; 546.05
• DOI: 10.1039/c8qi00705e
• Languages: English
• ISSNs: 2052-1553

Abstract

Abstract : A FRET based AceDAN–porphyrin(Zn) dyad was designed to generate red emission and singlet oxygen ( 1 O2 ) simultaneously, which were utilized successfully for two-photon excited fluorescence imaging and PDT of cancer cells. Abstract : The recent development of the two-photon technique offers a unique opportunity to improve the depth of imaging and treatment in photodynamic therapy (PDT). Herein, we designed a fluorescence resonance energy transfer (FRET) based AceDAN–porphyrin(Zn) dyad for two-photon excited fluorescence imaging and PDT of cancer cells simultaneously, in which the AceDAN moiety was selected as the two-photon absorption donor and the porphyrin(Zn) moiety served as the energy acceptor. Upon one-photon or two-photon excitation, the excited state energy of the AceDAN donor was transferred to the porphyrin(Zn) acceptor with high efficiency ( η EET = 98%), where red fluorescence and singlet oxygen were generated. Dyad1 exhibited high photocytotoxicity towards A549 cells (IC50 = 4.3 μM) with rapid cellular uptake while displaying low dark-cytotoxicity. Furthermore, by combining the advantages of two-photon excitation with a porphyrin(Zn) photosensitizer, the AceDAN–porphyrin(Zn) dyad has been successfully applied to A549 cells for imaging and photodynamic therapy under two-photon laser irradiation.