Enhancing the performance of LC-MS for intact protein analysis by counteracting the signal suppression effects of trifluoroacetic acid during electrospray. Issue 79 (21st August 2015)

• Record Type: Journal Article
• Authors: Chen, Jin; Liu, Zheyi; Wang, Fangjun; Mao, Jiawei; Zhou, Ye; Liu, Jing; Zou, Hanfa; Zhang, Yukui
• Is Part Of: Chemical communications. Volume 51:Issue 79(2015)
• Page Start: 14758
• Page End: 14760
• Publication Date: 2015-08-21
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cc06072a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : We develop an acidic vapor assisted electrospray ionization strategy within an enclosed electrospray ionization source to counteract the ion suppression effects caused by trifluoroacetic acid. Abstract : We develop an acidic vapor assisted electrospray ionization strategy within an enclosed electrospray ionization source to counteract the ion suppression effects caused by trifluoroacetic acid. The mass spectrometry signal intensity of intact proteins was improved 10 times and the number of valid signals for E. coli intact protein samples was improved 96% by using this strategy.