A turn-on fluorescent probe for tumor hypoxia imaging in living cells. Issue 79 (21st August 2015)

• Record Type: Journal Article
• Authors: Cai, Qi; Yu, Tao; Zhu, Weiping; Xu, Yufang; Qian, Xuhong
• Is Part Of: Chemical communications. Volume 51:Issue 79(2015)
• Page Start: 14739
• Page End: 14741
• Publication Date: 2015-08-21
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c5cc05518k
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : A turn-on fluorescent probe was designed and synthesized for tumor hypoxia imaging, which could show excellent reducing ability under chemical or bio-reductase conditions and good performance in hypoxia imaging for HeLa cells at different oxygen concentrations. Abstract : A novel "turn-on" fluorescent probeHP for hypoxia imaging was designed and synthesized based on rhodamine B and a naphthalimide fluorophore. The fluorescence ofHP is very weak owing to the FRET effect from rhodamine B to the azo-naphthalimide unit. Under hypoxia conditions, the azo-bond is reduced and the fluorescence at 581 nm enhances dramatically as a result of disintegration of the quencher structure. Verified by the cyclic voltammetry reduction potential and proposed productHPN, the probeHP could undergo the chemical and cytochrome P450 enzymatic reduction quickly. When cultured with HeLa cells, HP showed remarkable fluorescence differences at various oxygen concentrations, and the ratio of fluorescence intensity between hypoxic and normoxic cells could reach 9 fold.