Comparative Analysis of Ampicillin Plasma and Dried Blood Spot Pharmacokinetics in Neonates. Issue 1 (February 2018)

• Record Type: Journal Article
• Authors: Le, Jennifer; Poindexter, Brenda; Sullivan, Janice E.; Laughon, Matthew; Delmore, Paula; Blackford, Martha; Yogev, Ram; James, Laura P.; Melloni, Chiara; Harper, Barrie; Mitchell, Jeff; Benjamin, Daniel K.; Boakye-Agyeman, Felix; Cohen-Wolkowiez, Michael
• Is Part Of: Therapeutic drug monitoring. Volume 40:Issue 1(2018:Feb.)
• Publication Date: 2018-02
• Subjects: dried blood spot -- neonates -- ampicillin -- pharmacokinetic modeling; Pharmacokinetics -- Periodicals; Patient monitoring -- Periodicals; Drugs -- Analysis -- Periodicals; Body fluids -- Analysis -- Periodicals; Drug Therapy -- Periodicals; Monitoring, Physiologic -- Periodicals; Pharmacology -- Periodicals; 615.7
• DOI: 10.1097/FTD.0000000000000466
• Languages: English
• ISSNs: 0163-4356

Abstract

Abstract : Background: Dried blood spot (DBS) is a practical sampling strategy for pharmacokinetic studies in neonates. The utility of DBS to determine the population pharmacokinetics (pop-PK) of ampicillin, as well as accuracy versus plasma samples, was evaluated. Methods: An open-label, multicenter, opportunistic, prospective study was conducted in neonates. Ampicillin concentrations from plasma and DBS (CONCPlasma and CONCDBS ) were measured by liquid chromatographic tandem mass spectrometry and analyzed using pop-PK and statistical (including transformation) approaches. Results: A total of 29 paired plasma and DBS samples from 18 neonates were analyzed. The median (range) gestational age and postnatal age were 37 (27–41) weeks and 8 (1–26) days, respectively. The geometric mean of CONCDBS to CONCPlasma ratio was 0.56. Correlation analysis demonstrated strong association between CONCPlasma and CONCDBS ( r 2 = 0.902, analysis of variance P < 0.001). Using linear regression transformation, the estimated CONCPlasma (eCONCPlasma ) was derived using (CONCDBS − 3.223)/0.51. The median bias and geometric mean ratio improved to −11% and 0.88 (Wilcoxon signed-rank test, P < 0.001), respectively, when comparing eCONCPlasma to CONCPlasma . Furthermore, using pop-PK modeling, the median bias (interquartile range) for clearance and individual predicted concentrations improved to 8% (−11 to 50) and −8% (−34 to 11), respectively, when eCONCPlasma was used. Conclusions: After transformation, DBS sampling accurately predicted ampicillin exposure in neonates.