Phenyllactic acid production by simultaneous saccharification and fermentation of pretreated sorghum bagasse. (April 2015)

• Record Type: Journal Article
• Authors: Kawaguchi, Hideo; Teramura, Hiroshi; Uematsu, Kouji; Hara, Kiyotaka Y.; Hasunuma, Tomohisa; Hirano, Ko; Sazuka, Takashi; Kitano, Hidemi; Tsuge, Yota; Kahar, Prihardi; Niimi-Nakamura, Satoko; Oinuma, Ken-Ichi; Takaya, Naoki; Kasuga, Shigemitsu; Ogino, Chiaki; Kondo, Akihiko
• Is Part Of: Bioresource technology. Volume 182(2015)
• Page Start: 169
• Page End: 178
• Publication Date: 2015-04
• Subjects: AcCoA acetyl-CoA -- Aco aconitate -- ADP adenosine diphosphate -- AMP adenosine monophosphate -- AKG 2-oxoglutarate -- ATP adenosine triphosphate -- Cit citrate -- DHAP dihydroxyacetone phosphate -- E4P erythrose-4-phosphate -- EH enzymatic hydrolysis -- F6P fructose-6-phosphate -- FBP frucotose-1, 6-bisphosphate -- FPU filter paper unit -- Fum fumarate -- G6P glucose-6-phosphate -- GAP glyceraldehyde-3-phosphate -- GC–MS gas chromatography–mass spectrometry -- 4-HBA 4-hydroxybenzaldehyde -- 5-HMF 5-hydroxymethylfurfural -- HPLC high-performance liquid chromatography -- LC–MC/MS liquid chromatography–tandem quadrupole mass spectrometry -- Mal malate -- NAD+ and NADH oxidized and reduced nicotinamide adenine dinucleotide -- NADP+ and NADPH oxidized and reduced nicotinamide adenine dinucleotide phosphate -- OXA oxaloacetate -- PDH pyruvate dehydrogenase -- 6PG 6-phosphoglycerate -- PEP phosphoenolpyruvate -- PGA 2- or 3-phosphoglycerate -- Phe phenylalanine -- PhLA phenyllactic acid -- PPP pentose phosphate pathway -- Pyr pyruvate -- R5P ribose-5-phosphate -- Rib5P ribulose-5-phosphate -- S7P sedoheptulose-7-phosphate -- SHF separated hydrolysis and fermentation -- Shk shikimate -- SSF simultaneous saccharification and fermentation -- Suc succinate -- TCA tricarboxylic acid -- Trp tryptophan -- Tyr tyrosine -- X5P xylulose-5-phosphate; Sorghum -- Lignocellulosic biomass -- Phenyllactic acid -- Simultaneous saccharification and fermentation -- Fermentation inhibitor; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.01.097
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Phenyllactate was produced from dilute acid-pretreated sorghum bagasse by SSF. Sorghum bagasse yielded fermentation inhibitors of aldehyde and phenolic acid. Sorghum bagasse altered metabolic profiles during phenyllactate fermentation. SSF yielded 4.8-fold more phenyllactate than separate hydrolysis and fermentation. Abstract: Dilute acid-pretreated sorghum bagasse, which was predominantly composed of glucan (59%) and xylose (7.2%), was used as a lignocellulosic feedstock ford -phenyllactic acid (PhLA) production by a recombinant Escherichia coli strain expressing phenylpyruvate reductase from Wickerhamia fluorescens . During fermentation with enzymatic hydrolysate of sorghum bagasse as a carbon source, the PhLA yield was reduced by 35% compared to filter paper hydrolysate, and metabolomics analysis revealed that NAD(P)H regeneration and intracellular levels of erythrose-4-phosphate and phosphoenolpyruvate for PhLA biosynthesis markedly reduced. Compared to separate hydrolysis and fermentation (SHF) with sorghum bagasse hydrolysate, simultaneous saccharification and fermentation (SSF) of sorghum bagasse under glucose limitation conditions yielded 4.8-fold more PhLA with less accumulation of eluted components, including p -coumaric acid and aldehydes, which inhibited PhLA fermentation. These results suggest that gradual enzymatic hydrolysis during SSF enhances PhLA production under glucose limitation and reduces the accumulation of fermentation inhibitors, collectively leading to increased PhLA yield.