Editing plant genomes with CRISPR/Cas9. (April 2015)

• Record Type: Journal Article
• Authors: Belhaj, Khaoula; Chaparro-Garcia, Angela; Kamoun, Sophien; Patron, Nicola J; Nekrasov, Vladimir
• Is Part Of: Current opinion in biotechnology. Volume 32(2015)
• Page Start: 76
• Page End: 84
• Publication Date: 2015-04
• Subjects: Biotechnology -- Periodicals; 660.6
• DOI: 10.1016/j.copbio.2014.11.007
• Languages: English
• ISSNs: 0958-1669

Abstract

Graphical abstract: Highlights: Cas9 is an RNA-guided DNA endonuclease innate to prokaryotic immune systems. CRISPR/Cas9 has recently emerged as a powerful genome editing tool. CRISPR/Cas9 has been successfully applied in many organisms, including model and crop plants. CRISPR/Cas9 is a cheap, robust and easy to implement technology. Abstract : CRISPR/Cas9 is a rapidly developing genome editing technology that has been successfully applied in many organisms, including model and crop plants. Cas9, an RNA-guided DNA endonuclease, can be targeted to specific genomic sequences by engineering a separately encoded guide RNA with which it forms a complex. As only a short RNA sequence must be synthesized to confer recognition of a new target, CRISPR/Cas9 is a relatively cheap and easy to implement technology that has proven to be extremely versatile. Remarkably, in some plant species, homozygous knockout mutants can be produced in a single generation. Together with other sequence-specific nucleases, CRISPR/Cas9 is a game-changing technology that is poised to revolutionise basic research and plant breeding.