Fermentative hydrogen production using wheat flour hydrolysate by mixed culture. (13th April 2015)

• Record Type: Journal Article
• Authors: Han, Wei; Wang, Xiaonan; Ye, Lan; Huang, Jingang; Tang, Junhong; Li, Yongfeng; Ren, Nanqi
• Is Part Of: International journal of hydrogen energy. Volume 40:Number 13(2015)
• Page Start: 4474
• Page End: 4480
• Publication Date: 2015-04-13
• Subjects: Aspergillus awamori -- Fermentative hydrogen production -- Glucose concentration -- Solid-state fermentation -- Wheat flour hydrolysate; Hydrogen as fuel -- Periodicals; Hydrogène (Combustible) -- Périodiques; Hydrogen as fuel; Periodicals; 665.81
• DOI: 10.1016/j.ijhydene.2015.02.016
• Languages: English
• ISSNs: 0360-3199

Abstract

Abstract: Wheat bran was first used to produce glucoamylase by Aspergillus awamori from solid-state fermentation (SSF). Wheat flour with different mass ratios of 2%–8% (w/v) were hydrolyzed by glucoamylase to generate the wheat flour hydrolysates (containing glucose concentrations of 10.69–35.14 g/L) which were then utilized as substrate for fermentative hydrogen production by heat pretreated sludge. The cumulative hydrogen production increased from 1181.3 ml to 2379.6 ml as glucose concentration increased from 10.69 g/L to 35.14 g/L. The modified Gompertz model was used to describe the cumulative hydrogen production for different glucose concentrations. However, the maximum hydrogen yield of 1.9 mol H2 /mol glucose was observed at glucose concentration of 10.69 g/L probably due to the products inhibition and oxidization/reduction of NADH. The wheat flour hydrolysate could be used to replace commercial glucose for fermentative hydrogen production and therefore reduce the cost of hydrogen production for large scale. Highlights: Bran was utilized by Aspergillus awamori in solid-state fermentation to produce glucoamylase. Glucoamylase was used to hydrolyze wheat flour to obtain the generic feedstock. The feasibility of using wheat flour hydrolysate for H2 production was investigated. The highest cumulative hydrogen production of 2379.6 ml was observed.