Xylan hydrolysis in Populus trichocarpa × P. deltoides and model substrates during hydrothermal pretreatment. (March 2015)

• Record Type: Journal Article
• Authors: Trajano, Heather L.; Pattathil, Sivakumar; Tomkins, Bruce A.; Tschaplinski, Timothy J.; Hahn, Michael G.; Van Berkel, Gary J.; Wyman, Charles E.
• Is Part Of: Bioresource technology. Volume 179(2015)
• Page Start: 202
• Page End: 210
• Publication Date: 2015-03
• Subjects: 4M KOH PC 4 M KOH extraction post(P) sodium chlorite(C) extraction -- AG arabinogalactan -- AIR alcohol insoluble residue -- BX birchwood xylan -- DI deionized -- DP degree of polymerization -- ELISA enzyme-linked immunosorbent assay -- HC holocellulose -- HG homogalacturonan backbone -- HPLC high pressure liquid chromatography -- mAbs monoclonal antibodies -- m/z mass to charge ratio -- PtPd P.trichocarpa × P. deltoides -- RG-I/AG pectic-arabinogalactan -- UPLC–MS ultra-high performance liquid chromatography with mass spectrometry -- XG xyloglucans; Hydrolysis -- Hemicellulose -- Lignin–carbohydrate complex; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2014.11.090
• Languages: English
• ISSNs: 0960-8524

Abstract

Highlights: Hydrothermal pretreatment was performed in a flow-through reactor. Populus trichocarpa × P. deltoides, holocellulose, and birchwood xylan were used. Xylan hydrolysis was followed by glycome profiling and chromatography. Lignin–carbohydrate and xylan–cellulose bonds limited xylan hydrolysis. Cell wall structure strongly affected biomass deconstruction. Abstract: Previous studies defined easy and difficult to hydrolyze fractions of hemicellulose that may result from bonds among cellulose, hemicellulose, and lignin. To understand how such bonds affect hydrolysis, Populus trichocarpa × Populus deltoides, holocellulose isolated from P. trichocarpa × P. deltoides and birchwood xylan were subjected to hydrothermal flow-through pretreatment. Samples were characterized by glycome profiling, HPLC, and UPLC–MS. Glycome profiling revealed steady fragmentation and removal of glycans from solids during hydrolysis. The extent of polysaccharide fragmentation, hydrolysis rate, and total xylose yield were lowest for P. trichocarpa × P. deltoides and greatest for birchwood xylan. Comparison of results from P. trichocarpa × P. deltoides and holocellulose suggested that lignin–carbohydrate complexes reduce hydrolysis rates and limit release of large xylooligomers. Smaller differences between results with holocellulose and birchwood xylan suggest xylan–cellulose hydrogen bonds limited hydrolysis, but to a lesser extent. These findings imply cell wall structure strongly influences hydrolysis.