Enhancement of cell biomass and cell activity of astaxanthin-rich Haematococcus pluvialis. (June 2015)

• Record Type: Journal Article
• Authors: Sun, Han; Kong, Qing; Geng, Zhaoyan; Duan, Liangfei; Yang, Min; Guan, Bin
• Is Part Of: Bioresource technology. Volume 186(2015)
• Page Start: 67
• Page End: 73
• Publication Date: 2015-06
• Subjects: Haematococcus pluvialis -- Astaxanthin -- Cell biomass and cell activity -- Feeding model -- Mixed lights; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.02.101
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Astaxanthin of 91.8 ± 2.53 mg/L in a short time can be obtained with these methods. Dry cell weight of H. pluvialis by this feeding model is 2.0-fold of batch culture. Different responses of H. pluvialis at day and night were studied. Mixed lights were proved to be more effective on the transformation process. Abstract: Fed-batch culture and the transformation conditions of Haematococcus pluvialis in a 5 L photobioreactor were investigated. Methods of feeding model, low temperature at night and proper feeding time were used to increase both cell biomass and cell activity. Dry cell weight of 1.87 g/L which was 2.0-fold of batch culture and the specific growth rate of 0.43 d −1 suggested the superduper results of these methods to increase the dry cell weight in the short cultivation time. Furthermore, mixed lights of blue and white (ratio of 3:1) at 7000 xl were used to expedite the morphologic changes of H. pluvialis from green cells to red cyst cells with the yield of 91.8 ± 2.53 mg/L.