Effect of γ-irradiation on antioxidant and antiproliferative properties of oat β-glucan. (December 2015)

• Record Type: Journal Article
• Authors: Shah, Asima; Masoodi, F.A.; Gani, Adil; Ashwar, Bilal Ahmad
• Is Part Of: Radiation physics and chemistry. Volume 117(2015:Dec.)
• Page Start: 120
• Page End: 127
• Publication Date: 2015-12
• Subjects: γ-Irradiation -- Antioxidant activity -- Antiproliferative activity -- β-Glucan -- Oats; Radiation chemistry -- Periodicals; Radiometry -- Periodicals; Radiation -- Periodicals; Chimie sous rayonnement -- Périodiques; 539.2
• DOI: 10.1016/j.radphyschem.2015.06.022
• Languages: English
• ISSNs: 0969-806X

Abstract

Abstract: The present study was undertaken to examine effect of γ -rays on the antioxidant and antiproliferative potential of β- glucan isolated from oats. Irradiation doses of 0, 2, 6 and 10 kGy were given to extracted β- glucan. The samples were characterized by Fourier transform infrared spectroscopy, Gel permeation chromatography and quantitative estimation by (1-3) (1-4) β-d -Glucan assay kit (Megazyme). The average molecular weight of non-irradiated β -glucan was 199 kDa that decreased to 70 kDa at 10 kGy. Both FT-IR spectrum and chemical analysis revealed that the extracted β- glucan was pure having minor impurities. Antioxidant activity was evaluated by DPPH, lipid peroxidation, reducing power, metal chelating ability and oxidative DNA damage assays. Results revealed increased antioxidant activity of β -glucan with the increase in irradiation dose. Irradiated β -glucan also exhibited dose dependent cancer cell growth inhibition with irradiation doses. The study revealed that the low molecular weight β- glucan with increased antioxidant and antiproliferative activities can be produced by irradiation treatment. Highlights: Irradiation leads to depolymerization of oat β -glucan and reduced viscosity. Irradiated oat β -glucan exhibited increased antioxidant activity as compared to non-irradiated oat β -glucan. Irradiated oat β -glucan being more potent antiproliferative activity than non-irradiated β -glucan.