Interaction of p-benzoquinone with hemoglobin in smoker's blood causes alteration of structure and loss of oxygen binding capacity. (2016)

• Record Type: Journal Article
• Authors: Ghosh, Arunava; Banerjee, Santanu; Mitra, Amrita; Muralidharan, Monita; Roy, Bappaditya; Banerjee, Rajat; Mandal, Amit Kumar; Chatterjee, Indu B.
• Is Part Of: Toxicology reports. Volume 3(2016)
• Page Start: 295
• Page End: 305
• Publication Date: 2016
• Subjects: p-Benzoquinone -- Human smoker's hemoglobin -- Mass spectrometry -- Atomic force microscopy -- Oxygen binding capacity -- CORM-3 [tricarbonylchloro(glycinato)ruthenium(II)]; Toxicology -- Periodicals; Clinical toxicology -- Periodicals; Drug-Related Side Effects and Adverse Reactions; Hazardous Substances; Poisoning; Toxicology; Electronic journals; Periodicals; Periodicals; 571.9505
• DOI: 10.1016/j.toxrep.2016.02.001
• Languages: English
• ISSNs: 2214-7500

Abstract

Abstract: Cigarette smoke (CS) is an important source of morbidity and early mortality worldwide. Besides causing various life-threatening diseases, CS is also known to cause hypoxia. Chronic hypoxia would induce early aging and premature death. Continuation of smoking during pregnancy is a known risk for the unborn child. Although carbon monoxide (CO) is considered to be a cause of hypoxia, the effect of other component(s) of CS on hypoxia is not known. Here we show by immunoblots and mass spectra analyses that in smoker's blood p -benzoquinone ( p -BQ) derived from CS forms covalent adducts with cysteine 93 residues in both the β chains of hemoglobin (Hb) producing Hb- p -BQ adducts. UV–vis spectra and CD spectra analyses show that upon complexation with p -BQ the structure of Hb is altered. Compared to nonsmoker's Hb, the content of α-helix decreased significantly in smoker's Hb ( p = 0.0224). p -BQ also induces aggregation of smoker's Hb as demonstrated by SDS-PAGE, dynamic light scattering and atomic force microscopy. Alteration of Hb structure in smoker's blood is accompanied by reduced oxygen binding capacity. Our results provide the first proof that p -BQ is a cause of hypoxia in smokers. We also show that although both p -BQ and CO are responsible for causing hypoxia in smokers, exposure to CO further affects the function over and above that produced by Hb- p -BQ adduct.