Expression pattern of EYA4 in the common marmoset (Callithrix jacchus) cochlea. (1st January 2018)

• Record Type: Journal Article
• Authors: Matsuzaki, Saeko; Hosoya, Makoto; Okano, Hideyuki; Fujioka, Masato; Ogawa, Kaoru
• Is Part Of: Neuroscience letters. Volume 662(2018)
• Page Start: 185
• Page End: 188
• Publication Date: 2018-01-01
• Subjects: ADNSHL autosomal-dominant non-syndromic hearing loss -- DFNA10 non-syndromic sensorineural hearing loss -- EDTA ethylenediaminetetraacetic acid -- EYA eyes absent -- eya-HR highly conserved Eya domain -- eya-VR a variable Eya domain -- PBS phosphate-buffered saline -- SIX sine oculis homeobox; Eya4 -- Common marmoset -- Hereditary hearing loss -- Inner ear -- DFNA10 -- Sensorineural hearing loss; Neurology -- Periodicals; Neurology -- Periodicals; Research -- Periodicals; Neurologie -- Périodiques; Neuroanatomie -- Périodiques; Neuropharmacologie -- Périodiques; Neurophysiologie -- Périodiques; Neurology; Periodicals; Electronic journals; 617.48
• DOI: 10.1016/j.neulet.2017.10.030
• Languages: English
• ISSNs: 0304-3940

Abstract

Highlights: EYA4 immunoreactivity was observed in the adult common marmoset cochlea. EYA4 was co-localization with SIX1 in cochlear sensory and neuronal cells. EYA4 expression showed inter-species differences between primates and rodents. Abstract: The eyes absent (EYA) -like genes are essential for the formation of sensory organs among fly ( Drosophila melanogaster ) and mammals. EYA4, one of the vertebrate genes of Eya family, is reported to be causative for late-onset mid-frequency sensorineural hearing loss in humans, while Eya4 -deficient mice exhibited congenital profound deafness and otitis media with effusion due to the eustachian tube dysmorphology. Because of the species difference in the phenotype, the pathophysiology of EYA4 in the human cochlea has yet to be elucidated. Here, we examine the expression pattern of EYA4 in the cochlea of common marmoset ( Callithrix jacchus ), a non-human primate. The results indicated a distinct expression pattern of EYA4 in the adult marmoset cochleae, especially strong in all supporting cells, while in mouse their expressions were diminished. Interestingly, EYA4 expression in the hair cells, supporting cells and neurons was co-localized with sine oculis homeobox–SIX1, a transcription factor essential for the transcriptional activity of EYA4. The results revealed inter-species differences in the expression pattern of EYA4 gene in supporting cells between primates and rodents. The results also indicated a fundamental role of EYA4 in the primate auditory cells. Experiments with primate models such as marmosets or with human cochlear cells may provide cues about the unknown pathogenesis of EYA4 -related hearing loss.