Surface charge tunability as a powerful strategy to control electrostatic interaction for high efficiency silencing, using tailored oligopeptide-modified poly(beta-amino ester)s (PBAEs). (1st July 2015)

• Record Type: Journal Article
• Authors: Dosta, Pere; Segovia, Nathaly; Cascante, Anna; Ramos, Victor; Borrós, Salvador
• Is Part Of: Acta biomaterialia. Volume 20(2015)
• Page Start: 82
• Page End: 93
• Publication Date: 2015-07-01
• Subjects: Poly(beta-amino ester)s -- SiRNA -- Polyplexes -- Non viral systems -- Nanoparticles; Biomedical materials -- Periodicals; 610.28
• DOI: 10.1016/j.actbio.2015.03.029
• Languages: English
• ISSNs: 1742-7061

Abstract

Graphical abstract: Abstract: Here we present an extended family of pBAEs that incorporate terminal oligopeptide moieties synthesized from both positive and negative amino acids. Polymer formulations of mixtures of negative and positive oligopeptide-modified pBAEs are capable of condensing siRNA into discrete nanoparticles. We have demonstrated that efficient delivery of nucleic acids in a cell-type dependent manner can be achieved by careful control of the pBAE formulation. In addition, our approach of adding differently charged oligopeptides to the termini of poly(β-amino ester)s is of great interest for the design of tailored complexes having specific features, such as tuneable zeta potential. We anticipate that this surface charge tunability may be a powerful strategy to control unwanted electrostatic interactions, while preserving high silencing efficiency and reduced toxicity.