Development of an optimized broth enrichment culture medium for the isolation of Clostridium difficile. (December 2018)

• Record Type: Journal Article
• Authors: Connor, Mairéad C.; McGrath, John W.; McMullan, Geoff; Marks, Nikki; Fairley, Derek J.
• Is Part Of: Anaerobe. Volume 54(2018)
• Page Start: 92
• Page End: 99
• Publication Date: 2018-12
• Subjects: Clostridium difficile -- Culture media -- Growth -- Taurocholate -- Spores; Anaerobic infections -- Periodicals; Anaerobic bacteria -- Periodicals; Bacterial diseases -- Periodicals; Computer network resources; Anaerobic protozoa -- Periodicals; 579.3
• DOI: 10.1016/j.anaerobe.2018.08.006
• Languages: English
• ISSNs: 1075-9964

Abstract

Abstract: Clostridium difficile is a spore forming bacterium and the leading cause of colitis and antibiotic associated diarrhoea in the developed world. Effective recovery of spores, particularly in low numbers, is imperative to obtain accurate prevalence data, due to the low number of spores found within non-clinical samples (<20/ml). Through comparison of C. difficile enrichment media, this study showed the importance of selecting an effective enrichment media. Commonly used broths, such as Cooked Meat broth, promote significantly less growth than other available broths such as Brain Heart Infusion broth, BHI. The optimization of BHI using selective antibiotics, moxalactam and norfloxacin, and sodium taurocholate at a concentration of 0.4%, allowed for high growth rate (0.465 h -1 ), short lag times (<14 h) and recovery of spores at low concentrations. The optimized broth, designated BHIMN-T, out-performed other commonly used broths so can be recommended for future studies. Highlights: Comparison of enrichment media using qPCR to monitor growth of C. difficile. 0.4% sodium taurocholate provided optimum growth in Brain Heart Infusion with moxalactam and norfloxacin (BHIMN-T). BHIMN-T provided more effective growth of when compared to other commonly used growth media. BHIMN-T provided effective for the recovery of spores at low concentrations.