Impact of medicated feed along with clay mineral supplementation on Escherichia coli resistance to antimicrobial agents in pigs after weaning in field conditions. (October 2015)

• Record Type: Journal Article
• Authors: Jahanbakhsh, Seyedehameneh; Kabore, Kiswendsida Paul; Fravalo, Philippe; Letellier, Ann; Fairbrother, John Morris
• Is Part Of: Research in veterinary science. Volume 102(2015)
• Page Start: 72
• Page End: 79
• Publication Date: 2015-10
• Subjects: Pig -- E. coli -- Virulence gene -- Antimicrobial resistance -- Clay mineral (clinoptilolite); Veterinary medicine -- Periodicals; Veterinary Medicine -- Periodicals; Médecine vétérinaire -- Périodiques; Médecine vétérinaire -- Recherche -- Périodiques; Diergeneeskunde; 636.089
• DOI: 10.1016/j.rvsc.2015.07.014
• Languages: English
• ISSNs: 0034-5288

Abstract

Abstract: The aim of this study was to examine changes in antimicrobial resistance (AMR) phenotype and virulence and AMR gene profiles in Escherichia coli from pigs receiving in-feed antimicrobial medication following weaning and the effect of feed supplementation with a clay mineral, clinoptilolite, on this dynamic. Eighty E. coli strains isolated from fecal samples of pigs receiving a diet containing chlortetracycline and penicillin, with or without 2% clinoptilolite, were examined for antimicrobial resistance to 15 antimicrobial agents. Overall, an increased resistance to 10 antimicrobials was observed with time. Supplementation with clinoptilolite was associated with an early increase but later decrease in bla CMY− 2, in isolates, as shown by DNA probe. Concurrently, a later increase in the frequency of bla CMY−2 and the virulence genes iucD and tsh was observed in the control pig isolates, being significantly greater than in the supplemented pigs at day 28. Our results suggest that, in the long term, supplementation with clinoptilolite could decrease the prevalence of E. coli carrying certain antimicrobial resistance and virulence genes. Highlights: E. coli resistance to 10 antimicrobials in weaned pigs receiving medicated feed. Increase in frequency of bla CMY−2 and virulence genes iucD and tsh over time. Clinoptilolite associated with early increase but later decrease in bla CMY−2 . Clinoptilolite could reduce levels of antimicrobial resistant and virulent E. coli.