Temporal fluctuation in the abundance of alginate‐degrading bacteria in the gut of abalone Haliotis gigantea over 1 year. (14th March 2015)

• Record Type: Journal Article
• Authors: Tanaka, Reiji; Shibata, Toshiyuki; Miyake, Hideo; Mori, Tetsushi; Tamaru, Yutaka; Ueda, Mitsuyoshi; Bossier, Peter
• Is Part Of: Aquaculture research. Volume 47:Number 9(2016)
• Page Start: 2899
• Page End: 2908
• Publication Date: 2015-03-14
• Subjects: alginate -- abalone -- microbiota -- gut; Aquaculture -- Periodicals; Fishery management -- Periodicals; 639.8
• DOI: 10.1111/are.12740
• Languages: English
• ISSNs: 1355-557X

Abstract

Abstract: In this study, we identified and enumerated alginate‐degrading bacteria in the gut of abalone over 1‐year period. From a total of 360 colonies growing on agar medium enriched with alginate, 251 isolates (70%) had the ability to degrade alginate. In addition, a high number of viable alginate‐degrading bacteria were detected throughout the survey period. Alginate‐degrading bacteria were more abundant in the cold season relative to the summer season (10 7 vs. 10 4 CFU g −1, respectively). Strong positive correlation was also observed between the number of alginate‐degrading bacteria and feed intake ( R = 0.854; P < 0.01). The identified alginate‐degrading bacteria comprised of 35 species grouped into 11 genera including Algibacter, Formosa, Polarybacter, Tamlana, Tenacibaculum (CFB group), Roseobacter, Ruegeria, Silicibacter (α‐proteobacteria), Agarivorans, Shewanella and Vibrio (γ‐proteobacteria) respectively. More than 80% of the isolated alginate‐degrading bacteria belonged to the genus Vibrio, showing high homology to Vibrio cyclotorophicus, Vibrio splendidus, Vibrio halioticoli and Vibrio neonatus . Based on the results, it was suggested that algal‐polysaccharide (alginate) degrading bacteria (mainly Vibrio ) commonly exist in the gut of abalone and may play an important role in the degradation and digestion of the host's feed.