Characterization of digestive enzymes protease and alpha‐amylase activities in the thick‐lipped grey mullet (Chelon labrosus, Risso 1827). (16th April 2016)

• Record Type: Journal Article
• Authors: Pujante, Isabel Maria; Díaz‐López, Manuel; Mancera, Juan Miguel; Moyano, Francisco Javier
• Is Part Of: Aquaculture research. Volume 48:Number 2(2017)
• Page Start: 367
• Page End: 376
• Publication Date: 2016-04-16
• Subjects: Chelon labrosus -- proteases -- carbohydrases -- characterization -- sizes; Aquaculture -- Periodicals; Fishery management -- Periodicals; 639.8
• DOI: 10.1111/are.13038
• Languages: English
• ISSNs: 1355-557X

Abstract

Abstract: Activities of pepsin, trypsin, chymotrypsin, alpha‐amylase and lipase, as well as their optima and stability to pH and temperature, were determined in digestive extracts of thick‐lipped grey mullet, Chelon labrosus, of three different sizes: Group 1 (45.2 ± 3.0 g), Group 2 (180.9 ± 4.2 g), and Group 3 (328.5 ± 43.3 g). SDS‐PAGE zymograms were also used to assess the role of serine proteases in the digestive tract of C. labrosus . On the other hand, possible changes in the digestive enzyme profile of C. labrosus during development were observed, with a comparatively lower pepsin activity, higher activities of alkaline proteases and alpha‐amylase and no lipase activity recorded in pre‐adult specimens. It is suggested that these variations are linked to the changes in diet composition with age, moving from a partly carnivorous to a more herbivorous feeding habit.