Functional specialization of intestinal dendritic cell subsets during Th2 helminth infection in mice. Issue 1 (2nd November 2017)

• Record Type: Journal Article
• Authors: Redpath, Stephen A.; Heieis, Graham A.; Reynolds, Lisa A.; Fonseca, Nicolette M.; Kim, Sandra S.‐Y.; Perona‐Wright, Georgia
• Is Part Of: European journal of immunology. Volume 48:Issue 1(2018)
• Page Start: 87
• Page End: 98
• Publication Date: 2017-11-02
• Subjects: Alternative activation -- CD11b+ dendritic cells -- Helminth infection -- Programmed Death‐Ligand 2 -- Th2; Immunology -- Periodicals; 616.079
• DOI: 10.1002/eji.201747073
• Languages: English
• ISSNs: 0014-2980

Abstract

Abstract: Dendritic cells (DCs) are essential in dictating the nature and effectiveness of immune responses. In the intestine DCs can be separated into discrete subsets, defined by expression of CD11b and CD103, each with different developmental requirements and distinct functional potential. Recent evidence has shown that different intestinal DC subsets are involved in the induction of T helper (Th)17 and regulatory T cell responses, but the cells that initiate Th2 immune responses are still incompletely understood. We show that in the Th2 response to an intestinal helminth in mice, only CD11b + and not CD11b − DCs accumulate in the local lymph node, upregulate PDL2 and express markers of alternative activation. An enteric Th1 response instead activated both CD11b + and CD11b − DCs without eliciting alternative activation in either population. Functionally, only CD11b + DCs activated during helminth infection supported Th2 differentiation in naive CD4 + T cells. Together our data demonstrate that the ability to prime Th2 cells during intestinal helminth infection, is a selective and inducible characteristic of CD11b + DCs. Abstract : Distinct subsets of intestinal dendritic cells have been described, but it is not clear whether their different functions are fixed or flexible. Here CD11b+ DCs uniquely respond to helminth infection, upregulating PDL2, becoming alternatively activated, and driving Th2 differentiation. Th2 induction appears a selective and inducible characteristic of CD11b+ DCs.