Electrochemiluminescence Bioassays with a Water‐Soluble Luminol Derivative Can Outperform Fluorescence Assays. Issue 2 (5th December 2017)

• Record Type: Journal Article
• Authors: Mayer, Michael; Takegami, Shigehiko; Neumeier, Michael; Rink, Simone; Jacobi von Wangelin, Axel; Schulte, Silja; Vollmer, Moritz; Griesbeck, Axel G.; Duerkop, Axel; Baeumner, Antje J.
• Is Part Of: Angewandte Chemie international edition. Volume 57:Issue 2(2018)
• Page Start: 408
• Page End: 411
• Publication Date: 2017-12-05
• Subjects: biosensors -- electrochemiluminescence -- liposomes -- luminescence -- luminol; Chemistry -- Periodicals; 540
• DOI: 10.1002/anie.201708630
• Languages: English
• ISSNs: 1433-7851

Abstract

Abstract: The most efficient and commonly used electrochemiluminescence (ECL) emitters are luminol, [Ru(bpy)3 ] 2+, and derivatives thereof. Luminol stands out due to its low excitation potential, but applications are limited by its insolubility under physiological conditions. The water‐soluble m ‐carboxy luminol was synthesized in 15 % yield and exhibited high solubility under physiological conditions and afforded a four‐fold ECL signal increase (vs. luminol). Entrapment in DNA‐tagged liposomes enabled a DNA assay with a detection limit of 3.2 pmol L −1, which is 150 times lower than the corresponding fluorescence approach. This remarkable sensitivity gain and the low excitation potential establish m ‐carboxy luminol as a superior ECL probe with direct relevance to chemiluminescence and enzymatic bioanalytical approaches. Abstract : Let there be light : A highly water‐soluble luminol derivative ( m ‐carboxy luminol) was synthesized and shown to be an efficient electrochemiluminescence probe. Excellent performance was demonstrated in bioassays comprising a DNA hybridization assay with liposome signal enhancement.