A preliminary attempt to establish multiple stress response profiles of human skin fibroblasts exposed to mild or severe stress during ageing in vitro. (March 2018)

• Record Type: Journal Article
• Authors: Rattan, Suresh I.S.; Demirovic, Dino; Nizard, Carine
• Is Part Of: Mechanisms of ageing and development. Volume 170(2018)
• Page Start: 92
• Page End: 97
• Publication Date: 2018-03
• Subjects: Cellular aging -- Hormesis -- Fibroblasts -- Heat shock -- Serum starvation; Aging -- Periodicals; Developmental biology -- Periodicals; Aging -- Periodicals; Developmental Biology -- Periodicals; Vieillissement -- Périodiques; Biologie du développement -- Périodiques; Aging; Developmental biology; Periodicals; 612.67
• DOI: 10.1016/j.mad.2017.09.005
• Languages: English
• ISSNs: 0047-6374

Abstract

Highlights: Simultaneous analysis of the seven main pathways of stress response (SR) in normal human skin fibroblasts undergoing cellular ageing in vitro. Mild heat shock (HS) at 41 °C and severe HS at 43 °C given to early passage young cells have differential stress response profiles (SRP). Whereas mild HS activates most other SR pathways along with the lead HS response (HSR), severe HS results in the down regulation of most other pathways. Senescent cells are much more sensitive to the negative effects of HS. Mild serum starvation (2%) and severe serum starvation (0%) show age-related differences in SRP with lead response being autophagy. Abstract: Optimal stress response (SR) is an essential aspect of the property of dynamic homeostasis of all biological systems, including cells in culture. Whereas severe stress can induce the so-called stress-induced premature senescence (SIPS), a model developed by Olivier Toussaint, mild stress can strengthen homeodynamics and can postpone senescence through the phenomenon of hormesis. We have attempted to establish multiple stress response profiles (SRP) of early passage young and late passage senescent human facial skin fibroblasts, FSF-1, exposed to either mild (41 °C) and severe (43 °C) heat shock for 1 h, or to mild (2%) and severe (0%) serum deprivation for up to 48 h. The results obtained show that FSF-1 cells exposed to two different intensities of stress from two different stressors separately have differential SRP to mild and severe stress, which also vary significantly between young and senescent cells. Establishing multiple and differential SRP to mild and severe stress may facilitate distinguishing between the mild stress-induced beneficial hormetic effects and the harmful effects of severe stress.