Β‐carotene suppresses Porphyromonas gingivalis lipopolysaccharide‐mediated cytokine production in THP‐1 monocytes cultured with high glucose condition. (25th October 2017)

• Record Type: Journal Article
• Authors: Kajiura, Yukari; Nishikawa, Yasufumi; Lew, Jung Hwan; Kido, Jun‐ichi; Nagata, Toshihiko; Naruishi, Koji
• Is Part Of: Cell biology international. Volume 42:Number 1(2018)
• Page Start: 105
• Page End: 111
• Publication Date: 2017-10-25
• Subjects: β‐carotene -- diabetic complications -- glucose -- monocyte -- Porphyromonas gingivalis LPS; Cytology -- Periodicals; Cells -- Periodicals; 571.605
• DOI: 10.1002/cbin.10873
• Languages: English
• ISSNs: 1065-6995

Abstract

Abstract: Periodontitis is associated with development of diabetes mellitus. Although lipopolysaccharide (LPS) of Porphyromonas gingivalis (Pg), a major pathogen of periodontitis, may lead the progression of diabetes complications, the precise mechanisms are unclear. We, therefore, investigated the effects of β‐carotene on production of Pg LPS‐induced inflammatory cytokines in human monocytes cultured high glucose (HG) condition. THP‐1 cells were cultured under 5.5 mM or 25 mM glucose conditions, and cells were stimulated with Pg LPS. To investigate the productivity of TNF‐α, IL‐6, and MCP‐1, cell supernatants were collected for ELISA. To examine the effects of NF‐kB signals on cytokine production, Bay11‐7082 was used. HG enhanced Pg LPS‐induced production of TNF‐α, IL‐6, and MCP‐1 via NF‐kB signals in THP‐1. β‐carotene suppressed the enhancement of the Pg LPS ‐ induced cytokine production in THP‐1 via NF‐κB inactivation. Our results suggest that β‐carotene might be a potential anti‐inflammatory nutrient for circulating Pg LPS‐mediated cytokine production in diabetic patients with periodontitis.