The archaic roles of the lamprey NF-κB (lj-NF-κB) in innate immune responses. (December 2017)

• Record Type: Journal Article
• Authors: Su, Peng; Liu, Xin; Pang, Yue; Liu, Chang; Li, Ranran; Zhang, Qiong; Liang, Hongfang; Wang, Hao; Li, Qingwei
• Is Part Of: Molecular immunology. Volume 92(2017:Dec.)
• Page Start: 21
• Page End: 27
• Publication Date: 2017-12
• Subjects: NF-κB nuclear factor-kappa B -- RHD Rel homology domain -- ANKs ankyrin repeats -- NLS nuclear localization signals -- TAD transactivation domain -- GRR glycine-rich region -- IMD immune deficiency -- AMPs antimicrobial peptides -- IPT immunoglobulin-plexin-transcription -- ORF open reading frame -- IPTG isopropyl-1-thio-β-d-galactopyranoside -- BCA bicinchoninic acid -- EMSA electrophoretic mobility shift assay -- HRP hypothalamic regulatory peptides -- ECL electrochemical-luminescence -- FBS fetal bovine serum -- UTR untranslated region -- HMM hidden Markov models -- NJ neighbor-joining -- NTD N-terminal domain -- DimD dimerisation domain; Lamprey -- NF-κB -- Immune system -- Evolution; Immunochemistry -- Periodicals; Molecular biology -- Periodicals; Immunochemistry -- Periodicals; Allergy and Immunology -- Periodicals; Molecular Biology -- Periodicals; Immunochimie -- Périodiques; Biologie moléculaire -- Périodiques; Immunochemistry; Molecular biology; Periodicals; Electronic journals; 571.96
• DOI: 10.1016/j.molimm.2017.10.002
• Languages: English
• ISSNs: 0161-5890

Abstract

Highlights: lj-NF-κB gene was identified from lamprey after screening of a leukocytes cDNA library for the first time. We found lj-NF-κB could bind DNA and regulates genes expression. We found the ANKs of lj-NF-κB were the inhibition signal for the RHD of lj-NF-κB. Our research provides a new paradigm of the vertebrate NF-κB family origin. Abstract: The nuclear factor-kappa B (NF-κB) is a pleiotropic transcription factor regulating the expression of genes involved in various biological processes including the immune response and inflammation. Lamprey is regarded as a key species to provide meaningful clues for understanding the evolution of immune system; nevertheless, no information about lamprey NF-κB is reported. Thus, we have characterized a NF-κB homolog in lamprey (lj-NF-κB) for the deeper understanding of the role it played in lamprey immune system. The sequence and 3D structure analyses demonstrate that lj-NF-κB contained a Rel homology domain (RHD) and seven ankyrin repeats domains (ANKs), which would exhibit functional similarities to NF-κB superfamily proteins. This hypothesis was further proved by experiments. We found that the RHD of lj-NF-κB could interact with a mammalian κB response element, translocate to the nucleus to modulate gene ( IL-6, IL-1β and TNF-α ) expression, and the nuclear localization signals (NLS) was essential for the nuclear translocation. Furthermore, the ANKs of lj-NF-κB are the inhibition signal for the RHD of lj-NF-κB. The present results allow us to surmise that the lj-NF-κB should play a key role in immune response of lamprey, and the function of NF-κB has been maintained during evolution.