Bactericidal pathway of Escherichia coli in buffered saline treated with oxygen radicals. (16th March 2017)

• Record Type: Journal Article
• Authors: Kobayashi, Tsuyoshi; Iwata, Natsumi; Oh, Jun-Seok; Hahizume, Hiroshi; Ohta, Takayuki; Takeda, Keigo; Ishikawa, Kenji; Hori, Masaru; Ito, Masafumi
• Is Part Of: Journal of physics. Volume 50:Number 15(2017)
• Publication Date: 2017-03-16
• Subjects: oxygen radical -- bactericidal effect -- buffered saline -- hypochlorous acid; Physics -- Periodicals; 530
• DOI: 10.1088/1361-6463/aa61d7
• Languages: English
• ISSNs: 0022-3727

Abstract

Abstract: Bactericidal effects of phosphate buffered saline treated with electrically neutral oxygen radicals on Escherichia coli ( E. coli ) are studied using an atmospheric pressure radical source and colony counting method. To clarify the bactericidal mechanism, the chemistry in phosphate buffers treated with oxygen radicals with and without saline has been quantitatively investigated using the well-established chemical reporters N, N -diethyl-p-phenylenediamine reagent and Amplex Red for residual chlorine (HClO and ClO − ) and hydrogen peroxide (H2 O2 ), respectively. From the results, we have found that the presence of chlorine in the solutions treated with oxygen radicals is the most important factor in the further chemical reactions to generate hypochlorous acid in E. coli death, and H2 O2 is also linked to the bactericidal effect via an indirect chemical pathway.