Detection of Bacteria in Water with β-Galactosidase-Coated Magnetic Nanoparticles. (December 2018)

• Record Type: Journal Article
• Authors: Cui, Mingyue; Chang, Hao; Zhong, Yang; Wang, Min; Wu, Tianze; Hu, Xiao; Xu, Zhichuan J.; Xu, Chenjie
• Is Part Of: SLAS technology. Volume 23:Number 6(2018)
• Page Start: 624
• Page End: 630
• Publication Date: 2018-12
• Subjects: magnetic nanoparticles -- bacterial detection -- colorimetric assay; Medical laboratory technology -- Periodicals; Laboratories -- Equipment and supplies -- Periodicals; Diagnosis, Laboratory -- Periodicals; 616.075
• DOI: 10.1177/2472630318773407
• Languages: English
• ISSNs: 2472-6303

Abstract

Pathogenic contamination and resistant bacterial infections remain critical concerns in both developed and developing countries. Rapid and sensitive detection of pathogens is still a key requirement for both environmental and clinical settings. This article introduces a simple, colorimetric, cost-effective, and high-throughput system based on a positively charged iron oxide/enzyme complex for the detection of both gram-positive and gram-negative bacteria in water between 10 3 and 10 8 cfu/mL. This study provides an effective strategy for the identification and purification of pathogen contamination in drinking water.