Multiple-response optimization of the acidic treatment of the brown alga Ecklonia radiata for the sequential extraction of fucoidan and alginate. (December 2015)

• Record Type: Journal Article
• Authors: Lorbeer, Andrew John; Lahnstein, Jelle; Bulone, Vincent; Nguyen, Trung; Zhang, Wei
• Is Part Of: Bioresource technology. Volume 197(2015)
• Page Start: 302
• Page End: 309
• Publication Date: 2015-12
• Subjects: Response surface methodology -- Desirability function -- Experimental design -- Biorefinery -- Sulfated polysaccharides; Biomass -- Periodicals; Biomass energy -- Periodicals; Bioremediation -- Periodicals; Agricultural wastes -- Periodicals; Factory and trade waste -- Periodicals; Organic wastes -- Periodicals; Bioénergie -- Périodiques; Déchets agricoles -- Périodiques; Déchets industriels -- Périodiques; Déchets organiques -- Périodiques; Déchets (Combustible) -- Périodiques; 662.88
• DOI: 10.1016/j.biortech.2015.08.103
• Languages: English
• ISSNs: 0960-8524

Abstract

Graphical abstract: Highlights: Desirability functions were used to find the best compromise of responses. A high yield and molecular weight were achieved for alginate. The fucoidans were partially hydrolyzed during acidic extraction. Selectivity of sequential extractions improved with a lower pH acid treatment. A greater volume of extract could be recovered from the algae when using a lower pH. Abstract: The aim of this study was to optimize the acidic treatment of the brown alga Ecklonia radiata in order to extract fucoidan and facilitate the efficient sequential extraction of alginates. Response surface methodology was used to determine the effects of the temperature, pH, and duration of the acidic treatment on fucoidan yield, alginate extractability, and the molecular weight of sequentially extracted alginates. Desirability functions were then used to predict the best overall combinations of responses. The most desirable compromise allowed for the recovery of a fucoidan-rich fraction with a yield of 3.75% (w/w of alga) and the sequential extraction of alginates having an average molecular weight of 730 kDa at a yield of 44% (w/w of alga), with low cross-contamination between the products. The optimized acidic treatment could form the basis of an industrial biorefinery process for the production of both fucoidan and alginate.