A highly sensitive square wave voltammetry based biosensor for kinase activity measurements. Issue 5 (September 2015)

• Record Type: Journal Article
• Authors: Snir, Elza; Amit, Einav; Friedler, Assaf; Yitzchaik, Shlomo
• Is Part Of: Biopolymers. Volume 104:Issue 5(2015)
• Page Start: 515
• Page End: 520
• Publication Date: 2015-09
• Subjects: peptide monolayer -- kinase biosensor -- electrochemistry; Biopolymers -- Periodicals; Peptides -- Periodicals; Spectrum analysis -- Periodicals; 572.33
• DOI: 10.1002/bip.22653
• Languages: English
• ISSNs: 0006-3525

Abstract

ABSTRACT: An electrochemical biosensor has been developed for ultrasensitive, label‐free determination of protein kinase activity. The sensor is composed of a unique peptide monolayer on a gold electrode. It identifies the order change in the monolayer upon phosphorylation, via square wave voltametry (SWV) measurements. Disorder caused by the introduction of the phosphate groups onto the middle of the peptide sequence results in pinhole formation and therefore an increase in the electrochemical signal. The measured sensitivity was 100 n M of kinase and the dynamic range was 100 n M up to 11 μ M . Sensitivity was an order of magnitude higher, and the dynamic range wider by two orders of magnitude, as compared to our previously reported impedimetric method, in which the sensitivity was 1 μ M, and the dynamic range was 1–20 μ M . © 2015 Wiley Periodicals, Inc. Biopolymers (Pept Sci) 104: 515–520, 2015.