A facile approach to improve the spray time and stability of paper spray ionization mass spectrometry with a Teflon tube. Issue 46 (12th November 2018)

• Record Type: Journal Article
• Authors: Cao, Yuan; Huang, Guangming
• Is Part Of: Analytical methods. Volume 10:Issue 46(2018)
• Page Start: 5540
• Page End: 5546
• Publication Date: 2018-11-12
• Subjects: Chemistry, Analytic -- Periodicals; Analytical biochemistry -- Periodicals; Chemical laboratories -- Standards -- Periodicals; 543.1905
• DOI: 10.1039/c8ay01725e
• Languages: English
• ISSNs: 1759-9660

Abstract

Abstract : Improving the spray time and stability of paper spray ionization (PSI) mass spectrometry with a Teflon tube by reducing solvent evaporation. Abstract : Methanol plays a significant role in paper spray ionization mass spectrometry (PSI MS) analysis as a spray solvent. Nonetheless, using pure methanol as a spray solvent would lead to rapid evaporation of solvent and a short spray time. Herein, we present a facile approach to enable PSI to use pure volatile solvents with a Teflon tube, which could extend the spray time by reducing solvent evaporation in PSI MS analysis. An around three times longer spray time was found for PSI with a Teflon tube over conventional PSI. Other than longer spray time, the stability of PSI was found to be improved with a Teflon tube. The detection of a mixture of methylene blue and methyl violet consumed 20 μL methanol by PSI with a Teflon tube and 60 μL methanol by conventional PSI, and the MS analysis results of R bv ( R bv = ratio of methylene blue-to-methyl violet signal intensity) were consistent for PSI with a Teflon tube and conventional PSI. In addition, the RSDs for quantitative analysis were from 0.38 to 8.75% for PSI with a Teflon tube, which was illustrated via analysis of amitriptyline in blood and fetal bovine serum samples. This approach could provide a longer spray time and better stability for PSI, which could potentially benefit PSI MS analysis in various applications.