Identification of the actc1c cardiac actin gene in zebrafish. (October 2018)

• Record Type: Journal Article
• Authors: Ojehomon, Matiyo; Alderman, Sarah L.; Sandhu, Love; Sutcliffe, Sierra; Van Raay, Terry; Gillis, Todd E.; Dawson, John F.
• Is Part Of: Progress in biophysics and molecular biology. Volume 138(2018)
• Page Start: 32
• Page End: 37
• Publication Date: 2018-10
• Subjects: Actin protein -- Sequence conservation -- Actin orthologs -- In situ hybridization -- Zebrafish; Biophysics -- Periodicals; Biochemistry -- Periodicals; Biophysics -- Periodicals; Molecular Biology -- Periodicals; Biophysique -- Périodiques; Biochimie -- Périodiques; 571.4
• DOI: 10.1016/j.pbiomolbio.2018.06.007
• Languages: English
• ISSNs: 0079-6107

Abstract

Abstract: Zebrafish is rapidly becoming a key model organism for studying a variety of biological processes from molecules to organisms. Interactions involving actin, a contractile protein and part of the cytoskeleton, are regulated by actin binding proteins in the majority of physiological processes in eukaryotic cells. To understand the contribution of actin proteins to the physiological processes of zebrafish, it is important to identify the diverse isoforms of actin encoded by its genome; however, significant sequence identity complicates isoform assignments. Through a combination of human-directed sequence and functional analysis, we have assigned and performed localization of actc1c, a previously undesignated cardiac actin gene, and propose an updated assignment of α-actin protein isoform identities in zebrafish.