Pparα deficiency inhibits the proliferation of neuronal and glial precursors in the zebrafish central nervous system. Issue 12 (26th November 2018)

• Record Type: Journal Article
• Authors: Hsieh, Yen‐Che; Chiang, Ming‐Chang; Huang, Yin‐Cheng; Yeh, Tu‐Hsueh; Shih, Hung‐Yu; Liu, Han‐Fang; Chen, Hao‐Yuan; Wang, Chien‐Ping; Cheng, Yi‐Chuan
• Is Part Of: Developmental dynamics. Volume 247:Issue 12(2018)
• Page Start: 1264
• Page End: 1275
• Publication Date: 2018-11-26
• Subjects: Pparα -- neural development -- neuronal precursor -- glial precursor -- zebrafish; Morphogenesis -- Periodicals; Anatomy -- Periodicals; Anatomie -- Périodiques; Biologie du développement -- Périodiques; 571.833
• DOI: 10.1002/dvdy.24683
• Languages: English
• ISSNs: 1058-8388

Abstract

Abstract : Background : Many molecules and signaling pathways involved in neural development play a role in neurodegenerative diseases and brain tumor progression. Peroxisome proliferator‐activated receptor (PPAR) proteins regulate the differentiation of tissues and the progression of many diseases. However, the role of these proteins in neural development is unclear.Results : We examined the function of Pparα in the neural development of zebrafish. Two duplicate paralogs for mammalian PPARA / Ppara, namely pparaa and pparab, are present in the zebrafish genome. Both pparaa and pparab are expressed in the developing central nervous system in zebrafish embryos. Inhibiting the function of Pparα by using either the PPARα/Pparα antagonist GW6471 or pparaa or pparab truncated constructs produced identical phenotypes, which were sufficient to reduce the proliferation of neuronal and glial precursor cells without affecting the formation of neural progenitors.Conclusions : We demonstrated that both Pparαa and Pparαb proteins are essential regulators of the proliferation of neuronal and glial precursors. This study provides a better understanding of the functions of PPARα/Pparα in neural development and further expands our knowledge of the potential role of PPARα/Pparα in neurological disorders and brain tumors. Developmental Dynamics 247:1264–1275, 2018. © 2018 Wiley Periodicals, Inc. Key Findings: Zebrafish pparaa and pparab are expressed in the developing nervous system. Ppara deficiency reduces the proliferation of neuronal precursor cells. Ppara deficiency reduces the proliferation of glial precursors.