Effects of synthetic pyrethroids on RTG-2 cells. (2nd October 2018)

• Record Type: Journal Article
• Authors: Yurdakök-Dikmen, Begüm; Vejselova, Djanan; Kutlu, H. Mehtap; Filazi, Ayhan; Erkoç, Figen
• Is Part Of: Toxin reviews. Volume 37:Number 4(2018)
• Page Start: 304
• Page End: 312
• Publication Date: 2018-10-02
• Subjects: Synthetic pyrethroids -- RTG-2 -- cytotoxicity -- confocal microscopy -- mechanism of action; Toxicology -- Periodicals; Toxins -- Periodicals; 615.905
• DOI: 10.1080/15569543.2017.1366922
• Languages: English
• ISSNs: 1556-9543

Abstract

Abstract: The cellular and subcellular effects of some synthetic pyrethroids (permethrin, cypermethrin, tetramethrin, and deltamethrin) on RTG-2 trout cell line were assessed. Mitochondrial function/cell viability and membrane integrity were measured by MTT and LDH release assays. Major morphological changes as evaluated by light and confocal microscopy were treated cells had fragmented morphology and condensed nuclei, fragmentations with hole formation in cytoskeleton, pyknotic nuclei and micronucleus formation, shrinkage of the cells and nuclei. Generation of cytoplasmic vacuoles, prominent induction of cellular pleomorphism, and hydropic degeneration were recorded as compared with controls showing fusiform cells and central nucleus by H&E staining.