Investigation of binding properties of two ethidium derivatives with serum albumins: spectral and computational approach. Issue 12 (10th September 2018) - Akbay, Nuriye
Abstract : The interaction mechanisms of two ethidium derivatives, 3, 8-dibenzoylamino-5-ethyl-6-phenylphenantridinium chloride (E2) and 3, 8-diphenylacetylamino-5-ethyl-6-phenylphenantridinium chloride (E3) with serum albumins (BSA and HSA) have been investigated by a combined experimental and computational approach. Fluorescence quenching and UV–vis results revealed that the interaction of derivatives with albumins resulted in formation of ground-state complexes and the obtained Stern–Volmer quenching constants designate the presence of a static component in the quenching mechanisms. Thermodynamic parameters (ΔH and ΔS values) point out the ionic interactions play the major role in E2-BSA, E2-HSA and E3-HSA complexes. The van der Waals interactions are dominant forces in E3-BSA complex. Moreover, the obtained results in this study were supported with computational analyzes which have same tendency.