A fluorescence assay for the detection of hydrogen peroxide and hydroxyl radicals generated by metallonucleases. Issue 95 (14th November 2018)
- Record Type:
- Journal Article
- Title:
- A fluorescence assay for the detection of hydrogen peroxide and hydroxyl radicals generated by metallonucleases. Issue 95 (14th November 2018)
- Main Title:
- A fluorescence assay for the detection of hydrogen peroxide and hydroxyl radicals generated by metallonucleases
- Authors:
- Leichnitz, S.
Heinrich, J.
Kulak, N. - Abstract:
- Abstract : ROS quench assays for metal-based DNA cleavage show low selectivity and reliability – a fluorogenic assay was thus developed to reliably, selectively and sensitively detect H2 O2 and HO˙. Abstract : Metal complexes can initiate DNA cleavage by the formation of reactive oxygen species (ROS). A conventional assay to probe for ROS is to add quenchers in a gel electrophoresis experiment. As we show here, such an assay is neither selective nor reliable. Instead, we suggest the use of simple fluorogens, as tested here with several metallonucleases for the detection of H2 O2 and HO˙.
- Is Part Of:
- Chemical communications. Volume 54:Issue 95(2018)
- Journal:
- Chemical communications
- Issue:
- Volume 54:Issue 95(2018)
- Issue Display:
- Volume 54, Issue 95 (2018)
- Year:
- 2018
- Volume:
- 54
- Issue:
- 95
- Issue Sort Value:
- 2018-0054-0095-0000
- Page Start:
- 13411
- Page End:
- 13414
- Publication Date:
- 2018-11-14
- Subjects:
- Chemistry -- Periodicals
540 - Journal URLs:
- http://pubs.rsc.org/en/journals/journalissues/cc ↗
http://www.rsc.org/ ↗ - DOI:
- 10.1039/c8cc06996d ↗
- Languages:
- English
- ISSNs:
- 1359-7345
- Deposit Type:
- Legaldeposit
- View Content:
- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library DSC - 3139.350000
British Library DSC - BLDSS-3PM
British Library STI - ELD Digital store - Ingest File:
- 8863.xml