Azide-tagged sphingolipids for the proteome-wide identification of C16-ceramide-binding proteins. Issue 97 (20th November 2018)

• Record Type: Journal Article
• Authors: Schulte-Zweckel, Janine; Schneidewind, Tabea; Abad, Jose Luis; Brockmeyer, Andreas; Janning, Petra; Triola, Gemma
• Is Part Of: Chemical communications. Volume 54:Issue 97(2018)
• Page Start: 13742
• Page End: 13745
• Publication Date: 2018-11-20
• Subjects: Chemistry -- Periodicals; 540
• DOI: 10.1039/c8cc05691a
• Languages: English
• ISSNs: 1359-7345

Abstract

Abstract : Unknown ceramide-binding proteins can be identified by combining azide-tagged sphingolipids with MS-based proteomic profiling and protein array analysis. Abstract : Ceramide plays key roles in autophagy, inflammation and apoptosis. However, little is known about the molecular mechanisms regulating its function and only a handful of cellular effectors are known for this lipid. Here we show that azide-tagged sphingolipids are powerful tools to identify ceramide targets. The combination of a protein array analysis and a mass spectrometry-based proteomic profiling successfully detects known ceramide-binding proteins and identifies others not yet reported, several of which we validated using a variety of techniques.