Changes in duodenal CD163-positive cells in dogs with chronic enteropathy after successful treatment. Issue 7 (October 2018)

• Record Type: Journal Article
• Authors: Dandrieux, Julien RS; Martinez Lopez, Lina Maria; Stent, Andrew; Jergens, Albert; Allenspach, Karin; Nowell, Cameron J; Firestone, Simon M; Kimpton, Wayne; Mansfield, Caroline S
• Is Part Of: Innate immunity. Volume 24:Issue 7(2018:Oct.)
• Page Start: 400
• Page End: 410
• Publication Date: 2018-10
• Subjects: Canine -- CD163 -- chronic enteropathy -- inflammatory bowel disease -- macrophage; Natural immunity -- Periodicals; Endotoxins -- Periodicals; 616.07905
• DOI: 10.1177/1753425918799865
• Languages: English
• ISSNs: 1753-4259

Abstract

Chronic enteropathy (CE) in dogs is characterized retrospectively per treatment response as food-responsive enteropathy (FRE), antibiotic-responsive enteropathy (ARE), and immunosuppressant-responsive enteropathy (IRE) – the latter most resembling inflammatory bowel disease in people. The aim of this study was to characterize duodenal macrophages (Mϕ) in CE using immunohistochemistry; with calprotectin (CAL) as a marker of early differentiated Mϕ and CD163 expression as a marker for resident Mϕ in the duodenum before and after treatment. Prior to treatment, dogs with FRE and IRE had a lower CD163 + /CAL + ratio than control dogs (CTRL) in crypts; this increased significantly and normalized compared with CTRL after treatment. Conversely, the CD163 + /CAL + ratio in dogs with ARE was comparable to that in healthy dogs before and after treatment. In summary, these results suggest that Mϕ play a role in the pathogenesis of CE in FRE and IRE, with a decrease in resident Mϕ and an increase in early differentiated Mϕ, but not in ARE dogs. Mϕ normalize after successful treatment.