Emissive Synthetic Cofactors: Enzymatic Interconversions of tzA Analogues of ATP, NAD+, NADH, NADP+, and NADPH. Issue 4 (21st December 2017) - Hallé, François; Fin, Andrea; Rovira, Alexander R.; Tor, Yitzhak
Abstract: A series of enzymatic transformations, which generate visibly emissive isofunctional cofactors based on an isothiazolo[4, 3‐ d ]pyrimidine analogue of adenosine ( tz A ), was developed. Nicotinamide adenylyl transferase condenses nicotinamide mononucleotide and tz ATP to yieldN tz AD +, which can be enzymatically phosphorylated by NAD + kinase and ATP or tz ATP to the correspondingN tz ADP + . The latter can be engaged in NADP‐specific coupled enzymatic transformations involving conversion toN tz ADPH by glucose‐6‐phosphate dehydrogenase and reoxidation toN tz ADP + by glutathione reductase. TheN tz ADP + /N tz ADPH cycle can be monitored in real time by fluorescence spectroscopy. Abstract : Relatives in the spotlight : A series of enzymatic transformations generate visibly emissive isofunctional cofactors based on an isothiazolo[4, 3‐ d ]pyrimidine analogue of adenosine ( tz A, see figure). These synthetic cofactors may find utility as tools for monitoring redox reactions in vitro or potentially in living systems.