Photodynamic Therapy – In Vitro Investigation Using an Electrochemical Microsensor System. (2015)

• Record Type: Journal Article
• Authors: Marzioch, J.; Kieninger, J.; Sandvik, J.A.; Pettersen, E.O.; Peng, Q.; Urban, G.
• Is Part Of: Procedia engineering. Volume 120(2015)
• Page Start: 468
• Page End: 471
• Publication Date: 2015
• Subjects: Photodynamic therapy -- electrochemical sensor -- metabolic effects -- cancer -- cell respiration; Engineering -- Congresses; Engineering -- Periodicals; Engineering; Conference proceedings; Periodicals; 620.005
• DOI: 10.1016/j.proeng.2015.08.671
• Languages: English
• ISSNs: 1877-7058

Abstract

Abstract: A microsensor system to investigate photodynamic therapy of cancer cells in vitro was established. Monitoring of the cellular respiration during and after photodynamic therapy was demonstrated. The system comprises a sensor chip with cell culture chamber, allowing photodynamic treatment by laser light and simultaneous monitoring of metabolic parameter using electrochemical sensors in situ . The sensor chip is integrated into a fluorescence microscope allowing the detection of fluorescence emitted by the photosensitizer as well as to detect fluorescence from additional marker providing optical measurements in parallel to the microsensor readings.