Air-droplets as Gas Reservoir to Provide O2 to the Stored-Aqueous Droplets in Micro-channels. (2015)

• Record Type: Journal Article
• Authors: Akhtar, Mahmuda; Brandhoff, Lukas; van den Driesche, Sander; Vellekoop, Michael J.
• Is Part Of: Procedia engineering. Volume 120(2015)
• Page Start: 92
• Page End: 95
• Publication Date: 2015
• Subjects: droplet storage -- oxygen feeding -- fluorinated oil -- micro-fluidics; Engineering -- Congresses; Engineering -- Periodicals; Engineering; Conference proceedings; Periodicals; 620.005
• DOI: 10.1016/j.proeng.2015.08.573
• Languages: English
• ISSNs: 1877-7058

Abstract

Abstract: We present a method to supply oxygen to on-chip stored aqueous droplets surrounded by oxygen-permeable fluorinated oil in micro-channels. By generating air-droplets (air-bubbles) in between the aqueous droplets, oxygen diffuses to those aqueous droplets. This is confirmed by experiments comparing the effect of nitrogen bubbles and air-bubbles. The proposed method and device are a step towards long-term on-chip cultivation of mammalian cells and aerobic bacteria growth in droplets.