Cold-night responses in grapevine inflorescences. (October 2015)

• Record Type: Journal Article
• Authors: Sawicki, Mélodie; Ait Barka, Essaid; Clément, Christophe; Gilard, Françoise; Tcherkez, Guillaume; Baillieul, Fabienne; Vaillant-Gaveau, Nathalie; Jacquard, Cédric
• Is Part Of: Plant science. Volume 239(2015:Oct.)
• Page Start: 115
• Page End: 127
• Publication Date: 2015-10
• Subjects: AGPase glucose-1-phosphate adenyltransferase -- Ala alanine -- CEF cyclic electron flow -- DXPS X days post stress -- FNR ferredoxin-NADP + reductase -- GABA γ-aminobutyric acid -- Glu glutamate -- gs stomatal conductance -- Gewurtz. Gewurztraminer -- ISO isocitrate dehydrogenase NADP-like -- LEF linear electron flow -- Lys lysine -- NPQ non-photochemical quenching -- OXO 2-oxoglutarate deshydrogenase -- PC plastocyanin -- Pg gross photosynthesis -- Pn net photosynthesis -- Pinot Pinot noir -- Rd mitochondrial respiration -- TCA tricarboxylic acid cycle -- Y(I) effective photosystem I quantum yield -- Y(II) effective photosystem II quantum yield -- Y(NA) non-photochemical quantum yield of photosystem I due to acceptor side limitation -- Y(ND) non-photochemical quantum yield of photosystem I due to donor side limitation -- Y(NO) quantum yield of non-regulated energy dissipation in photosystem II -- Y(NPQ) quantum yield of regulated energy dissipation in photosystem II -- WBinv cell wall apoplastic invertase; Carbon metabolism -- Cold night -- Coulure -- Grapevine -- Inflorescences -- Photosynthetic activity; Botany -- Periodicals; Botanique -- Périodiques; 580
• DOI: 10.1016/j.plantsci.2015.07.023
• Languages: English
• ISSNs: 0168-9452

Abstract

Highlights: Chilling night leads to distinct responses depending on grapevine cultivars. Several metabolites are distinctly modulated in response to cold. The results explain partly the difference in cold susceptibility of used cultivars. Abstract: Cold nights impact grapevine flower development and fruit set. Regulation at the female meiosis stepmay be of considerable importance for further understanding on how flower reacts to cold stress. In this study, the impact of chilling temperature (0 °C overnight) on carbon metabolism was investigated in the inflorescencesof two cultivars, Pinot noir (Pinot) and Gewurztraminer (Gewurtz.). Fluctuations in photosynthetic activity and carbohydrate metabolism were monitored by analyzing gas exchanges, simultaneous photosystem I and II activities, andcarbohydrate content. Further, the expression of PEPc, PC, FNR, ISO, OXO, AGPase, amylases and invertase genes, activities of various enzymes, as well as metabolomic analysis were attained. Results showed that the chilling night has different impacts depending on cultivars. Thus, in Gewurtz., net photosynthesis ( P n ) was transiently increased whereas, in Pinot, the P n increase was persistent and concomitant with an inhibition of respiration. However, during the days following the cold night, photosynthetic activity was decreased, and the cyclic electron flow was inhibited in Gewurtz., suggesting lower efficient energy dissipation. Likewise, metabolomic analysis revealed that several metabolites contents (namely alanine, GABA, lysine and succinate)were distinctly modulated in the two cultivars. Taking together, these results reflect a photosynthetic metabolism alteration or internal CO2 conductance in Gewurtz. explaining partly why Pinot is less susceptible to cold stress.