Multilocus phylogeny and species delimitation within the genus Glauconycteris (Chiroptera, Vespertilionidae), with the description of a new bat species from the Tshopo Province of the Democratic Republic of the Congo. (22nd August 2017)

• Record Type: Journal Article
• Authors: Hassanin, Alexandre; Colombo, Raphaël; Gembu, Guy‐Crispin; Merle, Marie; Tu, Vuong Tan; Görföl, Tamás; Akawa, Prescott Musaba; Csorba, Gábor; Kearney, Teresa; Monadjem, Ara; Ing, Ros Kiri
• Is Part Of: Journal of zoological systematics and evolutionary research. Volume 56:Number 1(2018)
• Page Start: 1
• Page End: 22
• Publication Date: 2017-08-22
• Subjects: Africa -- DNA phylogeny -- evergreen forest -- morphology -- new species -- taxonomy; Animals -- Classification -- Periodicals; Zoology -- Periodicals; Evolution -- Periodicals; 578.012
• DOI: 10.1111/jzs.12176
• Languages: English
• ISSNs: 0947-5745

Abstract

Abstract: The genus Glauconycteris Dobson, 1875 currently contains 12 species of butterfly bats, all endemic to sub‐Saharan Africa. Most species are rarely recorded, with half of the species known from less than six geographic localities. The taxonomic status of several species remains problematic. Here, we studied the systematics of butterfly bats using both morphological and molecular approaches. We examined 45 adult specimens for external anatomy and skull morphology, and investigated the phylogeny of Glauconycteris using DNA sequences from three mitochondrial genes and 116 individuals, which in addition to outgroup taxa, included nine of the twelve butterfly bat species currently recognized. Four additional nuclear genes were sequenced on a reduced sample of 69 individuals, covering the outgroup and Glauconycteris species. Our molecular results show that the genus Glauconycteris is monophyletic, and that it is the sister‐group of the Asian genus Hesperoptenus . Molecular dating estimates based on either Cytb or RAG2 data sets suggest that the ancestor of Glauconycteris migrated into Africa from Asia during the Tortonian age of the Late Miocene (11.6–7.2 Mya), while the basal diversification of the crown group occurred in Africa at around 6 ± 2 Mya. The species G. superba is found to be the sister‐group of G. variegata, questioning its placement in the recently described genus Niumbaha . The small species living in tropical rainforests constitute a robust clade, which contains three divergent lineages: (i) the " poensis" group, which is composed of G. poensis, G. alboguttata, G. argentata, and G. egeria ; (ii) the " beatrix" group, which contains G. beatrix and G. curryae ; and (iii) the " humeralis" group, which includes G. humeralis and a new species described herein. In the " poensis" group, G. egeria is found to be monophyletic in the nuclear tree, but polyphyletic in the mitochondrial tree. The reasons for this mito‐nuclear discordance are discussed. Abstract : Here, we sequenced seven genes and examined morphological characters to investigate the systematics of Glauconycteris, an African genus of rarely recorded butterfly bat species. Our results support its monophyly, its association with Hesperoptenus, a sister‐group relationship between G. superba and G. variegata, and a group containing all small species from tropical rainforests. A new species is described. A robust mito‐nuclear discordance is observed for G. egeria . Our molecular dating estimates suggest a basal diversification at around 6 Mya. Glauconycteris superba (top, ©Alexandre Hassanin), Glauconycteris alboguttata (middle, ©Raphaël Colombo) and Glauconycteris sp. nov (bottom, ©Raphaël Colombo).