How to Build a Complex, Functional Propeller Protein, From Parts. Issue 4 (April 2016)

• Record Type: Journal Article
• Authors: Clark, Patricia L.
• Is Part Of: Trends in biochemical sciences. Volume 41:Issue 4(2016)
• Page Start: 290
• Page End: 292
• Publication Date: 2016-04
• Subjects: protein fold -- sequence evolution -- beta-propeller -- repeat protein structure; Biochemistry -- Periodicals; 572
• DOI: 10.1016/j.tibs.2016.02.010
• Languages: English
• ISSNs: 0968-0004

Abstract

Abstract : By combining ancestral sequence reconstruction and in vitro evolution, Smock et al. identified single motifs that assemble into a functional five-bladed β-propeller, and a likely route for conversion into the more complex, extant single chain fusion. Interestingly, although sequence diversification destabilized five-motif fusions, it also destabilized aggregation-prone intermediates, increasing the level of functional protein in vivo .