Emissive Synthetic Cofactors: Enzymatic Interconversions of tzA Analogues of ATP, NAD+, NADH, NADP+, and NADPH. (21st December 2017)

• Record Type: Journal Article
• Authors: Hallé, François; Fin, Andrea; Rovira, Alexander R.; Tor, Yitzhak
• Is Part Of: Angewandte Chemie. Volume 130:Number 4(2018)
• Page Start: 1099
• Page End: 1102
• Publication Date: 2017-12-21
• Subjects: Kinasen -- Kofaktoren -- NAD+ -- NADP+ -- Nukleotide; Chemistry -- Periodicals; 540
• DOI: 10.1002/ange.201711935
• Languages: English
• ISSNs: 0044-8249

Abstract

Abstract: A series of enzymatic transformations, which generate visibly emissive isofunctional cofactors based on an isothiazolo[4, 3‐ d ]pyrimidine analogue of adenosine ( tz A ), was developed. Nicotinamide adenylyl transferase condenses nicotinamide mononucleotide and tz ATP to yieldN tz AD +, which can be enzymatically phosphorylated by NAD + kinase and ATP or tz ATP to the correspondingN tz ADP + . The latter can be engaged in NADP‐specific coupled enzymatic transformations involving conversion toN tz ADPH by glucose‐6‐phosphate dehydrogenase and reoxidation toN tz ADP + by glutathione reductase. TheN tz ADP + /N tz ADPH cycle can be monitored in real time by fluorescence spectroscopy.