Neuroanatomical localization of nitric oxide synthase (nNOS) in the central nervous system of carp, Labeo rohita during post-embryonic development. Issue 46 (November 2015)

• Record Type: Journal Article
• Authors: Biswas, Saikat P.; Jadhao, Arun G.; Bhoyar, Rahul C.; Palande, Nikhil V.; Sinh, Dharmendra P.
• Is Part Of: International journal of developmental neuroscience. Issue 46(2015:Nov.)
• Page Start: 14
• Page End: 26
• Publication Date: 2015-11
• Subjects: CEB cerebellum -- CNS central nervous system -- CSF cerebrospinal fluid -- Dc central zone of dorsal telencephalic area -- Dd dorsal zone of dorsal telencephalic area -- Dl lateral zone of dorsal telencephalic area -- Dm medial zone of dorsal telencephalic area -- En entopeduncular nucleus -- ggl ganglionic layer of cerebellum -- Hb habenula -- IMR intermediate reticular formation -- IP interpeduncular nucleus -- IRF inferior reticular formation -- MB mammillary body -- MC mauthner cell -- Mlf medial longitudinal fascicle -- mol molecular layer of cerebellum -- NLT lateral tuberal nucleus -- Nmlf nucleus of medial longitudinal fascicle -- NPOm nucleus preopticus magnocellularis -- NPOp nucleus preopticus parvocellularis -- NPP periventricular preoptic nucleus -- NR reticular nucleus -- nXm motor nucleus of vagal nerve -- OB olfactory bulb -- ON octaval nucleus -- OT optic tectum -- PGa anterior preglomerular nucleus -- POA preoptic area -- PVO paraventitricular organ -- SC suprachaismatic nucleus -- SFGS stratum fibrossum et griesum superficiale -- SO stratum opticum -- SPV stratum periventriculare -- SR superior reticular formation -- TBS tris buffered saline -- Tl torus longitudinalis -- V ventricle -- Vd dorsal nucleus of ventral telencephalic area -- Vp posterior nucleus of ventral telencephalic area -- Vv ventral nucleus of ventral telencephalic area; Nitric oxide -- nNOS immunocytochmeistry -- Carp -- Central nervous system -- Development; Developmental neurobiology -- Periodicals; Neurology -- Periodicals; Neurologie du développement -- Périodiques; Developmental neurobiology; Periodicals; 612.8
• DOI: 10.1016/j.ijdevneu.2015.06.004
• Languages: English
• ISSNs: 0736-5748

Abstract

Highlights: First report showing nNOS immunoreactivity in L. rohita during ontogeny. Only in adult, nNOS was localized in the nucleus preopticus magnocellularis (NPOm). No nNOS labeling was noted in the entopeducular nucleus and Mauthner cells of adult. Present study results differ with goldfish where nNOS was not localized in NPOm. Abstract: Nitric oxide (NO) is a chemically diffusible molecular messenger playing various roles in both vertebrates and invertebrates. Nitric oxide synthase (NOS) is the key enzyme in synthesis of NO. The neuroanatomical distribution pattern of neuronal nitric oxide synthase (nNOS) was studied and developing stages of Labeo rohita such as hatchlings (10–15 mm), frys (15–35 mm), semi-fingerlings (35–65 mm), fingerlings (65–100 mm) and adults (350–370 mm) were used. In the telencephalon, nitrergic cells were observed in both pallial and subpallial regions along with entopeduncular nucleus suggesting the involvement of NO in the control of sensory functions throughout the development. In the diencephalon, nNOS positive neurons were localized in the nucleus preopticus periventricularis and preopticus parvocellularis throughout development while nucleus preopticus magnocellularis was found immunopositive only in adult specimens who suggest the involvement of NO in the hormonal regulation. nNOS immunoreaction was also noted in suprachaismatic nucleus, habenula, lateral tuberal nucleus, paraventricular organ and anterior division of preglomerular nucleus throughout development. In the mesencephalic region, nNOS immunoreactivity was seen in the optic tectum, torus longitudinalis, nucleus of median longitudinal fascicle and occulomotor nucleus indicate the role of NO in integration of visual inputs and modulates motor control of the eyes and movements. Caudally, in the rhombencephalon, the cerebellum, the nucleus reticularis, the octaval nucleus and the motor nucleus of vagal nerve were nNOS positive during development. nNOS reactive cells and fibers were noted in the spinal motor column, thus suggesting a role of NO in gestation and startle response from early development.