Association analysis of exome variants and refraction, axial length, and corneal curvature in a European–American population. Issue 12 (11th September 2018)

• Record Type: Journal Article
• Authors: Vergara, Candelaria; Bomotti, Samantha M.; Valencia, Cristian; Klein, Barbara E.K.; Lee, Kristine E.; Klein, Ronald; Klein, Alison P.; Duggal, Priya
• Is Part Of: Human mutation. Volume 39:Issue 12(2018)
• Page Start: 1973
• Page End: 1979
• Publication Date: 2018-09-11
• Subjects: axial length -- corneal curvature -- exome array -- Haplotype Reference Consortium -- imputation -- refraction -- spherical equivalent; Human chromosome abnormalities -- Periodicals; Mutation (Biology) -- Periodicals; 616.04205
• DOI: 10.1002/humu.23628
• Languages: English
• ISSNs: 1059-7794

Abstract

Abstract: Refractive errors, myopia, and hyperopia are common visual disorders greatly affecting older individuals. Refraction is determined by genetic factors but only a small percentage of its variation has been explained. We performed a genetic association analysis with three ocular phenotypes: spherical equivalent (a continous measure of refraction), axial length, and corneal curvature in 1, 871 European–Americans from the Beaver Dam Eye Study. Individuals were genotyped on the Illumina exome array and imputed to the Haplotype Reference Consortium reference panel. After increasing the number of analyzed variants in targeted protein‐coding regions 10‐fold via imputation, we confirmed associations for two previously known loci with corneal curvature (chr4q12, rs2114039; g.55092626T > C, β = −0.03 (95% confidence interval [CI]): −0.06, −0.01, P value = 0.01) and spherical equivalent (chr15q14, rs634990; g.35006073T > C, β = −0.27, 95% CI: −0.45, −0.09, P value = 3.79 × 10 −3 ). Despite increased single nucleotide polymorphism (SNP) density, we did not detect any novel significant variants after correction for multiple comparisons. In summary, we confirmed two previous loci associated with corneal curvature and spherical equivalent in a European–American population highlighting the potential biological role of those regions in these traits. Abstract : Refractive errors myopia, and hyperopia are common visual disorders greatly affecting older individuals. Refraction are genetically determined but only a small percentage of its variation has been explained. We performed a exome genetic association analysis with refraction, axial length, and corneal curvature in European–Americans, confirming two previous loci associated with corneal curvature (chr4q12) and spherical equivalent (chr15q14) in a European–American population Americans from the Beaver Dam Eye Study.