Isolation and genomic characterization of gosling gout caused by a novel goose astrovirus. Issue 6 (19th June 2018)

• Record Type: Journal Article
• Authors: Yang, Jing; Tian, Jiajun; Tang, Yi; Diao, Youxiang
• Is Part Of: Transboundary and emerging diseases. Volume 65:Issue 6(2018)
• Page Start: 1689
• Page End: 1696
• Publication Date: 2018-06-19
• Subjects: astrovirus -- goslings -- gout -- next‐generation sequencing; Veterinary medicine -- Periodicals; 636.089
• DOI: 10.1111/tbed.12928
• Languages: English
• ISSNs: 1865-1674

Abstract

Abstract: A severe infectious disease characterized with gout, haemorrhage and swellings of kidneys has affected goslings around the major goose‐producing regions in China since November 2016. A Novel goose‐origin astrovirus (AStV), designated as AStV/SDPY/Goose/1116/17 (AStV‐SDPY) strain, was isolated from diseased goslings, and experimental reproduction of gout was successful using the AStV‐SDPY strain. Additionally, the AStV‐SDPY was conducted for its full genome sequencing characterization using next‐generation sequencing (NGS) technique on Illumina HiSeq platform. A complete genome of the AStV‐SDPY was 7, 252 nt in length and encoded three viral proteins. Phylogenetic analysis revealed that AStV‐SDPY strain belongs to an independent branch of avian astroviruses, and the nucleotide homology among AStV‐SDPY and other classic avian astrovirus strains was only 48.8%–68.2%. Results of above data indicated the causative agent of the gosling gout occurring in China is a novel divergent goose astrovirus.