Metabolic profiling of listeria rhombencephalitis in small ruminants by 1H high‐resolution magic angle spinning NMR spectroscopy. (17th October 2018)

• Record Type: Journal Article
• Authors: Precht, Christina; Diserens, Gaëlle; Vermathen, Martina; Oevermann, Anna; Lauper, Josiane; Vermathen, Peter
• Is Part Of: NMR in biomedicine. Volume 31:Number 12(2018)
• Page Start: n/a
• Page End: n/a
• Publication Date: 2018-10-17
• Subjects: goats -- HR‐MAS -- Listeria monocytogenes -- neurolisteriosis -- sheep; Nuclear magnetic resonance -- Periodicals; Magnetic Resonance Spectroscopy -- Periodicals; 574
• DOI: 10.1002/nbm.4023
• Languages: English
• ISSNs: 0952-3480

Abstract

Abstract : Listeria rhombencephalitis is caused by infection with Listeria monocytogenes and is associated with a high mortality rate in humans and ruminants. Little is known about the metabolic changes associated with neurolisteriosis in particular and infectious central nervous system (CNS) diseases in general. The purpose of our study was to investigate the metabolic changes associated with listeria rhombencephalitis in small ruminants (goats and sheep) as a model for inflammatory CNS disease by 1 H high‐resolution magic angle spinning nuclear magnetic resonance ( 1 H HR‐MAS NMR) spectroscopy of brain biopsies obtained from the brainstem and thalamus. Statistical analysis revealed distinct differences in the metabolic profile of brainstem biopsies, the primary location of listeria rhombencephalitis with moderate or severe inflammatory changes. N ‐Acetylaspartate (NAA), N ‐acetylaspartylglutamate, choline, myo‐inositol and scyllo‐inositol were decreased, and glycine, phosphocholine, taurine and lactate were increased, in the diseased group ( n = 13) in comparison with the control group ( n = 12). In the thalamus, which showed no or only mild inflammatory changes in the majority of animals, no statistically significant metabolic changes were observed. However, trends for metabolic alterations were partly the same as those found in the brainstem, including NAA, choline and lactate. This may be an indicator of metabolic changes occurring in the early stages of the disease. Therefore, further research with a larger number of animals is needed to evaluate the presence of subtle metabolic changes associated with mild inflammatory changes in the thalamus. In conclusion, 1 H HR‐MAS NMR investigation of listeria rhombencephalitis identified brain metabolite changes, offering new insights into the disease pathophysiology. Abstract : In this metabolomics study, neurolisteriosis was investigated using brain biopsies of goats and sheep, a natural animal model. The partial least squares‐discriminant analysis (PLS‐DA) model discriminated between control and diseased cases in the brainstem, the primary location of the disease. Metabolite alterations are compatible with neuronal necrosis, membrane breakdown and brain edema. In the morphologically unaffected or mildly affected thalamus, no discrimination between control and diseased cases was found, but a trend involving partly the same metabolites as in the brainstem was seen.