Mechanistic insights into geniposide regulation of bile salt export pump (BSEP) expression. Issue 65 (5th November 2018)

• Record Type: Journal Article
• Authors: Wu, Guixin; Wen, Min; Sun, Lin; Li, Huitao; Liu, Yubei; Li, Rui; Wu, Feihua; Yang, Rong; Lin, Yining
• Is Part Of: RSC advances. Volume 8:Issue 65(2018)
• Page Start: 37117
• Page End: 37128
• Publication Date: 2018-11-05
• Subjects: Chemistry -- Periodicals; 540.5
• DOI: 10.1039/c8ra06345a
• Languages: English
• ISSNs: 2046-2069

Abstract

Abstract : PGC-1α and CARM1 interact with FXR to increase FXR-dependent BSEP expression upon GE treatment. Abstract : Geniposide (GE) is a major component isolated from Gardenia jasminoides Ellis, which has been used to treat cholestasis liver diseases. Our previous study has shown that GE could notably increase mRNA and protein expressions of BSEP in cholestatic rats. BSEP plays a critical role in maintenance of the enterohepatic circulation of bile acids. BSEP could be regulated by the transactivation pathway of farnesoid X receptor (FXR) and nuclear factor erythroid 2-related factor (Nrf2). Here the mechanisms for BSEP regulation by GE were investigated. GE induced the mRNA levels of BSEP in HepG2 cells and cholestatic mice, and knockdown of FXR and Nrf2 reduced the mRNA expression of BSEP at varying degrees after treatment of GE. FXR acts as the major regulator of BSEP transcription. The involvement of FXR regulated BSEP expression by GE was further investigated. An enhancement was observed in FXR-dependent BSEP promoter activation using luciferase assay. ChIP assay further confirmed the interaction between FXR and BSEP after GE treatment. Using siRNA and ChIP assays, we demonstrated that peroxisome-proliferator-activated receptor γ co-activator-1α (PGC-1α) and co-activator-associated arginine methyltransferase 1 (CARM1) were predominantly recruited to the BSEP promoter upon FXR activation by GE. In conclusion, GE regulated the expression of BSEP through FXR and Nrf2 signaling pathway. The FXR transactivation pathway was enhanced by increasing recruitment of coactivators PGC-1α and CARM1 upon GE treatment, coupled with an increased binding of FXR to the BSEP promoter.