A ratiometric fluorescent BODIPY-based probe for rapid and highly sensitive detection of cysteine in human plasma. Issue 23 (15th October 2018)

• Record Type: Journal Article
• Authors: Wang, Nannan; Wang, Yue; Gao, Jinhua; Ji, Xin; He, Jinling; Zhang, Jian; Zhao, Weili
• Is Part Of: Analyst. Volume 143:Issue 23(2018)
• Page Start: 5728
• Page End: 5735
• Publication Date: 2018-10-15
• Subjects: Chemistry, Analytic -- Periodicals; 543
• DOI: 10.1039/c8an01438h
• Languages: English
• ISSNs: 0003-2654

Abstract

Abstract : The probe could respond to Cys/Hcy, exhibiting a ratiometric fluorescence response through intramolecular displacement with the amino group displacing the sulfur function to form amino-substituted BODIPY. Abstract : Biological thiols, especially low molecular weight thiols, including cysteine (Cys), homocysteine (Hcy) and glutathione (GSH), play a pivotal role in physiological and pathological systems. Thus, the detection of biothiols is highly important for early diagnosis of diseases and evaluation of disease progression. Herein, we developed a highly selective and sensitive ratiometric fluorescent 8-Cl BODIPY-based probe with high fluorescence quantum yields. The probe displayed a sensitive response to Cys and Hcy over other biothiols, which can be visualized colorimetrically and/or fluorescently. The probe was successfully applied to detect Cys in human plasma, demonstrating its great value for practical application in biological sample analysis.